CTRL-Z was never actually an End-Of-File character in MS-DOS.

You've come to this page because you've said something similar to the following:

Character 26, CTRL-Z, is the End-Of-File (EOF) character in MS-DOS/PC-DOS/DR-DOS.

This is the Frequently Given Answer to such statements.

MS-DOS didn't have an End-Of-File character of any sort. The MS-DOS system API from version 2.0 onwards treated files as simple octet streams, with no particular octet values having any special meanings. The end-of-file position of a file was recorded in file metadata. (It's the length field in the file's directory entry.) No special meaning was ascribed to character 26 (or indeed to any other character) within file data.

All of the so-called "text file" semantics that are conventionally, but erroneously, ascribed to MS-DOS were in fact artifacts of the C libraries for C compilers targetting DOS. The conversion of CR+LF sequences into just LF was done by the C libraries. The handling of character 26 was done by the C libraries. None of this was actually behaviour inherent in DOS itself.

One can even see this for onesself:

DOS makes no distinction between "text" files and "binary" files in its system API. Files are, to DOS, simple octet streams, with no such division. The DOS API function is INT 0x21 with AX=0x3f, which, as can be seen, does not treat any characters in a file specially, nor perform any translation of the characters in a file. DOS itself is actually a lot more like Unix in this regard than many people think.

Ironically, this greater similarity to Unix was hidden by language libraries, even though several of those language implementations attempted to give Unix-like semantics to DOS as much as they could. This is particularly ironic for DJGPP, for example.

The treatment of character 26 and the handling of "text" files was a shared delusion, common to the C libraries and the code of many programs that ran on top of DOS, from the aforementioned COPY command to text editors. It was wholly layered above DOS itself.

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